Journal: Brain and Behavior

Article Title: RTMS Mitigates Simulated Spaceflight‐Induced Emotional Dysfunction Through VEGFR‐2‐Mediated Neuroprotection

doi: 10.1002/brb3.71799

Figure Lengend Snippet: SU5416 inhibited the expression of VEGFR‐2 in the prefrontal cortex. a, b. Schematic diagram of SSCE (a) and rTMS (b) application. (c) Schematic of the paradigm. (d) Representative western blot bands using total protein extracts from the PFC region. (e) Quantification of protein expression levels in the PFC among the CON, SSCE + rTMS, and SSCE + rTMS + SU5416 groups (from four mice in each group).

Article Snippet: The membranes were incubated at 4°C overnight with GAPDH (1:1000, GeneTex, GTX100118) and VEGFR‐2 (1:1000, Proteintech, 26415‐1‐AP).

Techniques: Expressing, Western Blot

Journal: Brain and Behavior

Article Title: RTMS Mitigates Simulated Spaceflight‐Induced Emotional Dysfunction Through VEGFR‐2‐Mediated Neuroprotection

doi: 10.1002/brb3.71799

Figure Lengend Snippet: Verification of the expression of molecules related to neuroprotection. a, b. Representative images (a) and statistical results (b) showing the VEGFR‐2 immunofluorescence, respectively, scale bar, 50 µm, n = 3 mice. c, d. Representative images (c) and statistical results (d) showing the NeuN immunofluorescence, respectively, scale bar, 50 µm, n = 3 mice. e, f. Representative images (e) and statistical results (f) showing the SMI312 immunofluorescence, respectively, scale bar, 50 µm, n = 3 mice.

Article Snippet: The membranes were incubated at 4°C overnight with GAPDH (1:1000, GeneTex, GTX100118) and VEGFR‐2 (1:1000, Proteintech, 26415‐1‐AP).

Techniques: Expressing, Immunofluorescence

Journal: Brain and Behavior

Article Title: RTMS Mitigates Simulated Spaceflight‐Induced Emotional Dysfunction Through VEGFR‐2‐Mediated Neuroprotection

doi: 10.1002/brb3.71799

Figure Lengend Snippet: Summary of rTMS‐induced behavioral rescue through prefrontal VEGFR‐2 signaling under simulated spaceflight conditions. Simulated space composite environment (SSCE) exposure triggers emotional dysfunction and social deficits. Therapeutic intervention with repetitive transcranial magnetic stimulation (rTMS) activates VEGFR‐2‐dependent neuroprotection, restoring neuronal integrity and ameliorating depressive‐ and anxiety‐like behaviors. Notably, pharmacological blockade of VEGFR‐2 abolishes these beneficial effects, supporting the involvement of this pathway for rTMS efficacy in extreme environments.

Article Snippet: The membranes were incubated at 4°C overnight with GAPDH (1:1000, GeneTex, GTX100118) and VEGFR‐2 (1:1000, Proteintech, 26415‐1‐AP).

Techniques: Transcranial Magnetic Stimulation

Journal: Annals of Hematology

Article Title: Reduced number of endothelial progenitor cells in adult patients with beta thalassemia major

doi: 10.1007/s00277-026-06979-1

Figure Lengend Snippet: Violin plot representations of the proportion of ( a ) VEGFR-2 + CD34 + cells and ( b ) VEGFR-2 + CD133 + cells in TM patients (“TM”) ( n = 25) vs. healthy controls (“Controls”) ( n = 11)

Article Snippet: Briefly: isolated cells were stained with FITC-labeled VEGFR-2 (vascular endothelial growth factor receptor-2), a key endothelial lineage marker (R&D, Minneapolis, USA), CY5.5 labeled CD45 (Dako, Denmark), and either PE labeled CD133 or CD34 (Miltenyi Biotech, Auburn, CA, USA).

Techniques:

Journal: Annals of Hematology

Article Title: Reduced number of endothelial progenitor cells in adult patients with beta thalassemia major

doi: 10.1007/s00277-026-06979-1

Figure Lengend Snippet: Violin plot showing the proportion of VEGFR-2 + CD34 + cells in TM patients with a history of thromboembolic events ( n = 6) vs. TM patients with no history of thromboembolic events ( n = 19)

Article Snippet: Briefly: isolated cells were stained with FITC-labeled VEGFR-2 (vascular endothelial growth factor receptor-2), a key endothelial lineage marker (R&D, Minneapolis, USA), CY5.5 labeled CD45 (Dako, Denmark), and either PE labeled CD133 or CD34 (Miltenyi Biotech, Auburn, CA, USA).

Techniques: